Microfluidic Protein Separation by Free Flow Isoelectric Focusing
نویسندگان
چکیده
Disposable, inexpensive microfluidic devices have the potential to become a robust tool for proteomic research for separating and identifying proteins and protein complexes. In this work, a preparative scale free flow IEF isoelectric focusing (FFIEF) device was designed, investigated, and optimized. A constant electric field of up to 350V/cm was generated across the separation channel upon optimization. Devivce characteristics, such as the pH gradient of the outlet fraction, heat dispersion efficiency were also characterized. A complex biological sample of whole cell lysate was separated on the microdevice and further evaluated by subsequent 2D gel electrophoresis of particular fractions.
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تاریخ انتشار 2010